Difference between revisions of "Part:BBa K103014"

Latest revision as of 14:18, 28 October 2008

OmpA_linker_alpha_linker

It's fusion of E. coli outer membrane protein (OmpA) domain ( BBa_K103006) with β-lactamase subunit alpha ( BBa_K103008)
Fusion partners are connected via Gly-Ser-Gly linker
It's base for creation of hunter fusions in 'hunter and prey' selection system
Hunter protein should be attached via the second linker (using BamHI+ Biobrick standard suffix enzyme). It will be exported outside the cell and attached to the outer membrane
Interaction between hunter and prey (fused with BBa_K103020) makes cells ampicillin resistant
This part uses our NdeI/SacI/BamHI fusion cloning substandard

This protein structure was predicted using threading and protein fold prediction and may differ from actual one.

Sequence and Features

Assembly Compatibility:

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 __NOTOC__
 <partinfo>BBa_K103014 short</partinfo>
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-<!-- Add more about the biology of this part here
+<!-- Add more about the biology of this part here -->
+{|
+|
 ===Usage and Biology===
+*It's fusion of E. coli outer membrane protein (OmpA) domain ([[Part:BBa_K103006 | BBa_K103006]]) with &beta;-lactamase subunit alpha ([[Part:BBa_K103008 | BBa_K103008]])
+*Fusion partners are connected via Gly-Ser-Gly linker
+*It's base for creation of hunter fusions in 'hunter and prey' selection system
+*Hunter protein should be attached via the second linker (using BamHI+ Biobrick standard suffix enzyme). It will be exported outside the cell and attached to the outer membrane
+*Interaction between hunter and prey (fused with [[Part:BBa_K103020 | BBa_K103020]]) makes cells ampicillin resistant
+*This part uses our NdeI/SacI/BamHI fusion cloning substandard
+|[[Image:Ompalinkalfa.jpg|250px]]
+This protein structure was predicted using threading and protein fold prediction and may differ from actual one.
+|}
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