Difference between revisions of "Part:BBa K103001:Design"

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Preparation of BBa_K103001 is in details described [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=5&arg0=1_October_2008&arg1=6_October_2008&arg2=7_October_2008&arg3=8_October_2008&arg4=9_October_2008&name=Preparation%20of%20AID(BBa_K103001) here] (entries from Univeristy of Warsaw 2008 iGEM team notebook).
 
Preparation of BBa_K103001 is in details described [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=5&arg0=1_October_2008&arg1=6_October_2008&arg2=7_October_2008&arg3=8_October_2008&arg4=9_October_2008&name=Preparation%20of%20AID(BBa_K103001) here] (entries from Univeristy of Warsaw 2008 iGEM team notebook).
  
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AID-coding fragment was amplified from [http://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-AID pMPM-T5-AID] vector using primers: 5' AAACTGCAGCGGCCGCTACTAGTAAAGCTTTCAAAGTCCCAAAGTACGAAATG  3' and 5' TATCTAGAACCATGGACAGCCTCTTG 3'. Resulting fragment was digested with XbaI and PstI and cloned into standard BioBrick vector ([https://parts.igem.org/wiki/index.php?title=Part:pSB1A3 pSB1A3]
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AID-coding fragment was amplified from [http://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-AID pMPM-T5-AID] vector using primers: 5' AAACTGCAGCGGCCGCTACTAGTATCAAAGTCCCAAAGTACGAAATG 3' and 5' TATCTAGAACCATGGACAGCCTCTTG 3'. Resulting fragment was digested with XbaI and PstI and cloned into standard BioBrick vector ([https://parts.igem.org/wiki/index.php?title=Part:pSB1A3 pSB1A3])
  
 
===Source===
 
===Source===
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Human cDNA (plasmid Trc99a with AID from publication below)
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Human cDNA (plasmid pTrc99a with AID obtained from Svend Petersen-Mahrt, DNA Editing Laboratory, Cancer Research UK, Clare Hall Laboratories, South Mimms Hert EN6 3LD, United Kingdom)
  
 
===References===
 
===References===
 
Ref. [http://www.ncbi.nlm.nih.gov/pubmed/12097915?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum]
 
Ref. [http://www.ncbi.nlm.nih.gov/pubmed/12097915?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum]

Latest revision as of 13:59, 28 October 2008

AID protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 78
    Illegal SapI site found at 179


Design Notes

Preparation of BBa_K103001 is in details described [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=5&arg0=1_October_2008&arg1=6_October_2008&arg2=7_October_2008&arg3=8_October_2008&arg4=9_October_2008&name=Preparation%20of%20AID(BBa_K103001) here] (entries from Univeristy of Warsaw 2008 iGEM team notebook).

AID-coding fragment was amplified from [http://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-AID pMPM-T5-AID] vector using primers: 5' AAACTGCAGCGGCCGCTACTAGTATCAAAGTCCCAAAGTACGAAATG 3' and 5' TATCTAGAACCATGGACAGCCTCTTG 3'. Resulting fragment was digested with XbaI and PstI and cloned into standard BioBrick vector (pSB1A3)

Source

Human cDNA (plasmid pTrc99a with AID obtained from Svend Petersen-Mahrt, DNA Editing Laboratory, Cancer Research UK, Clare Hall Laboratories, South Mimms Hert EN6 3LD, United Kingdom)

References

Ref. [http://www.ncbi.nlm.nih.gov/pubmed/12097915?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum]