Difference between revisions of "Part:BBa K3394003"
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<partinfo>BBa_K3394003 short</partinfo> | <partinfo>BBa_K3394003 short</partinfo> | ||
| − | GS linker represents a glycine-serine linker, which is used for the separation of two domains in such way that they are physically connected but the original | + | GS linker represents a glycine-serine linker, which is used for the separation of two domains in such way that they are physically connected but the original functionality remains. |
We decided to include GS linker in our design to keep the catalytic domain of endo5a separate from the cellulose-binding domain (CBD). | We decided to include GS linker in our design to keep the catalytic domain of endo5a separate from the cellulose-binding domain (CBD). | ||
Revision as of 16:47, 17 October 2020
Glycine-serine (GS) linker
GS linker represents a glycine-serine linker, which is used for the separation of two domains in such way that they are physically connected but the original functionality remains.
We decided to include GS linker in our design to keep the catalytic domain of endo5a separate from the cellulose-binding domain (CBD).
We chose this specific 10 amino-acid linker (GGGGSGGGGS) because it was used in a previous study to fuse endo5a to another domain.
NCBI Genbank number for the GS linker sequence linked to the cellulase endo5a is HQ657205.1.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]