Difference between revisions of "Part:BBa K3394006:Design"

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===References===
 
===References===
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The Autodisplay Story, from Discovery to Biotechnical and Biomedical Applications.
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Jose, J., & Meyer, T. F. (2007). The Autodisplay Story, from Discovery to Biotechnical and Biomedical Applications. Microbiology and Molecular Biology Reviews, 71(4), 600–619.
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Joachim Jose and Thomas F. Meyer
+
 
 +
Amir, L., Carnally, S. A., Rayo, J., Rosenne, S., Melamed Yerushalmi, S., Schlesinger, O., ... & Alfonta, L. (2013). Surface display of a redox enzyme and its site-specific wiring to gold electrodes. Journal of the American Chemical Society, 135(1), 70-73.

Revision as of 15:36, 17 October 2020


AIDA-I auto-display system


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 241
    Illegal EcoRI site found at 937
    Illegal EcoRI site found at 4139
    Illegal PstI site found at 289
    Illegal PstI site found at 875
    Illegal PstI site found at 3387
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 241
    Illegal EcoRI site found at 937
    Illegal EcoRI site found at 4139
    Illegal PstI site found at 289
    Illegal PstI site found at 875
    Illegal PstI site found at 3387
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 241
    Illegal EcoRI site found at 937
    Illegal EcoRI site found at 4139
    Illegal BamHI site found at 1139
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 241
    Illegal EcoRI site found at 937
    Illegal EcoRI site found at 4139
    Illegal PstI site found at 289
    Illegal PstI site found at 875
    Illegal PstI site found at 3387
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 241
    Illegal EcoRI site found at 937
    Illegal EcoRI site found at 4139
    Illegal PstI site found at 289
    Illegal PstI site found at 875
    Illegal PstI site found at 3387
    Illegal NgoMIV site found at 1351
    Illegal NgoMIV site found at 1398
    Illegal NgoMIV site found at 1766
    Illegal NgoMIV site found at 1926
    Illegal NgoMIV site found at 2280
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 3213


Design Notes

We designed the passenger genes' sequence to express the cellulolytic complex.


Source

Artificial sequence - Synthetic construct

References

Jose, J., & Meyer, T. F. (2007). The Autodisplay Story, from Discovery to Biotechnical and Biomedical Applications. Microbiology and Molecular Biology Reviews, 71(4), 600–619.

Amir, L., Carnally, S. A., Rayo, J., Rosenne, S., Melamed Yerushalmi, S., Schlesinger, O., ... & Alfonta, L. (2013). Surface display of a redox enzyme and its site-specific wiring to gold electrodes. Journal of the American Chemical Society, 135(1), 70-73.