Difference between revisions of "Part:BBa K3394004"
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Our cellulolytic complex includes cellulase endo5a linked to CBD (cellulose binding domain) through a GS-linker and with a 6-histidine tag (His-tag) at the N-terminus.
 
Our cellulolytic complex includes cellulase endo5a linked to CBD (cellulose binding domain) through a GS-linker and with a 6-histidine tag (His-tag) at the N-terminus.
  
−
We made another design in which the gene order is different: first the CBD and then the cellulase endo5a.
+
In this design the order of genes is: first the cellulase endo5a and then CBD.
 +
We made another design in which the gene order is different: first the CBD and then the cellulase endo5a (BBa_K3394005).
  
  

Revision as of 14:52, 17 October 2020


Cellulolytic complex [1]: His-Endo5a-GS-CBD

Since cellulose cannot cross the membrane, we engineered E. coli to express cellulolytic complex on its surface through AIDA-1 auto-display system.

Our cellulolytic complex includes cellulase endo5a linked to CBD (cellulose binding domain) through a GS-linker and with a 6-histidine tag (His-tag) at the N-terminus.

In this design the order of genes is: first the cellulase endo5a and then CBD. We made another design in which the gene order is different: first the CBD and then the cellulase endo5a (BBa_K3394005).



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 988
    Illegal EcoRI site found at 1314
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 988
    Illegal EcoRI site found at 1314
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 988
    Illegal EcoRI site found at 1314
    Illegal BamHI site found at 28
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 988
    Illegal EcoRI site found at 1314
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 988
    Illegal EcoRI site found at 1314
    Illegal AgeI site found at 1289
  • 1000
    COMPATIBLE WITH RFC[1000]