Difference between revisions of "Part:BBa K3373001:Design"
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===References=== | ===References=== | ||
| + | 1. Crozet P, Navarro FJ, Willmund F, Mehrshahi P, Bakowski K, Lauersen KJ, Pérez-Pérez ME, Auroy P, Gorchs Rovira A, Sauret-Gueto S, Niemeyer J, Spaniol B, Theis J, Trösch R, Westrich LD, Vavitsas K, Baier T, Hübner W, de Carpentier F, Cassarini M, Danon A, Henri J, Marchand CH, de Mia M, Sarkissian K, Baulcombe DC, Peltier G, Crespo JL, Kruse O, Jensen PE, Schroda M, Smith AG, Lemaire SD. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth Biol. 2018 Sep 21;7(9):2074-2086. doi: 10.1021/acssynbio.8b00251. Epub 2018 Sep 5. PMID: 30165733. | ||
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| + | 2. Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S. A modular cloning system for standardized assembly of multigene constructs. PLoS One. 2011 Feb 18;6(2):e16765. doi: 10.1371/journal.pone.0016765. PMID: 21364738; PMCID: PMC3041749. | ||
| + | |||
| + | 3. de Souza ML, Wackett LP, Boundy-Mills KL, Mandelbaum RT, Sadowsky MJ. Cloning, characterization, and expression of a gene region from Pseudomonas sp. strain ADP involved in the dechlorination of atrazine. Appl Environ Microbiol. 1995 Sep;61(9):3373-8. doi: 10.1128/AEM.61.9.3373-3378.1995. PMID: 7574646; PMCID: PMC167616. | ||
Latest revision as of 22:14, 16 October 2020
atzA P. sp. ADP codon optimized for C. reinhardtii
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 825
Illegal PstI site found at 1389 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 825
Illegal PstI site found at 1389 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 825
Illegal PstI site found at 1389 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 825
Illegal PstI site found at 1389
Illegal NgoMIV site found at 253
Illegal NgoMIV site found at 653 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The CDS is standardized in the Phytobrick standard for the C. reinhardtii MoClo Kit at the B3-B4 position by adding BbsI sites and the corresponding fusion sites on both ends.
The stop codon was removed to allow for tagging at the C-terminal position, the stop codon being provided by the part on the position B5.
Source
Pseudomonas sp. ADP strain.
References
1. Crozet P, Navarro FJ, Willmund F, Mehrshahi P, Bakowski K, Lauersen KJ, Pérez-Pérez ME, Auroy P, Gorchs Rovira A, Sauret-Gueto S, Niemeyer J, Spaniol B, Theis J, Trösch R, Westrich LD, Vavitsas K, Baier T, Hübner W, de Carpentier F, Cassarini M, Danon A, Henri J, Marchand CH, de Mia M, Sarkissian K, Baulcombe DC, Peltier G, Crespo JL, Kruse O, Jensen PE, Schroda M, Smith AG, Lemaire SD. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth Biol. 2018 Sep 21;7(9):2074-2086. doi: 10.1021/acssynbio.8b00251. Epub 2018 Sep 5. PMID: 30165733.
2. Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S. A modular cloning system for standardized assembly of multigene constructs. PLoS One. 2011 Feb 18;6(2):e16765. doi: 10.1371/journal.pone.0016765. PMID: 21364738; PMCID: PMC3041749.
3. de Souza ML, Wackett LP, Boundy-Mills KL, Mandelbaum RT, Sadowsky MJ. Cloning, characterization, and expression of a gene region from Pseudomonas sp. strain ADP involved in the dechlorination of atrazine. Appl Environ Microbiol. 1995 Sep;61(9):3373-8. doi: 10.1128/AEM.61.9.3373-3378.1995. PMID: 7574646; PMCID: PMC167616.