Difference between revisions of "Part:BBa K3610051:Design"
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===Design Notes=== | ===Design Notes=== | ||
Chosing an appropriate way to link the CORE ectodomain with the SmallBit NanoLuc sequence required a short sequence with small amino acids that allowes in frame translation. The flag tag is necessary for western blotting. | Chosing an appropriate way to link the CORE ectodomain with the SmallBit NanoLuc sequence required a short sequence with small amino acids that allowes in frame translation. The flag tag is necessary for western blotting. | ||
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===Source=== | ===Source=== | ||
Revision as of 22:12, 16 October 2020
CORE ectodomain / SmallBit NanoLuc for S. cerevisiae
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1740
Illegal BamHI site found at 373
Illegal BamHI site found at 1765
Illegal BamHI site found at 2117 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Chosing an appropriate way to link the CORE ectodomain with the SmallBit NanoLuc sequence required a short sequence with small amino acids that allowes in frame translation. The flag tag is necessary for western blotting.
Source
BBa_K2809002, BBa_K3610010, BBa_M10019, BBa_K416001, BBa_K3610016
References
England, Christopher G.; Ehlerding, Emily B.; Cai, Weibo (2016): NanoLuc: A Small Luciferase Is Brightening Up the Field of Bioluminescence. In: Bioconjug Chem 27 (5), S. 1175–1187. DOI: 10.1021/acs.bioconjchem.6b00112.
Wang, Lei; Albert, Markus; Einig, Elias; Fürst, Ursula; Krust, Damaris; Felix, Georg (2016): The pattern-recognition receptor CORE of Solanaceae detects bacterial cold-shock protein. In: Nature plants 2, S. 16185. DOI: 10.1038/nplants.2016.185.