Difference between revisions of "Part:BBa K103019"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K103019 short</partinfo> | <partinfo>BBa_K103019 short</partinfo> | ||
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| − | <!-- Add more about the biology of this part here | + | <!-- Add more about the biology of this part here --> |
===Usage and Biology=== | ===Usage and Biology=== | ||
| + | *Construct for creating prey fusions for our 'hunter and prey' selection system | ||
| + | *Fusion partners are connected via Gly-Ser-Gly linker | ||
| + | *Prey protein should be attached via linker (using BamHI+ Biobrick standard suffix enzyme). | ||
| + | *Fusion protein can be purified on NiNTA bead (tkanks to presence of N-terminal His-Tag). | ||
| + | *Generator under control of T7 promoter | ||
| + | *Interaction between hunter and prey added to the medium makes cells ampicillin resistant | ||
| + | *This part uses our NdeI/SacI/BamHI fusion cloning substandard | ||
| + | |||
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Latest revision as of 12:54, 28 October 2008
alpha_linker under PT7
Usage and Biology
- Construct for creating prey fusions for our 'hunter and prey' selection system
- Fusion partners are connected via Gly-Ser-Gly linker
- Prey protein should be attached via linker (using BamHI+ Biobrick standard suffix enzyme).
- Fusion protein can be purified on NiNTA bead (tkanks to presence of N-terminal His-Tag).
- Generator under control of T7 promoter
- Interaction between hunter and prey added to the medium makes cells ampicillin resistant
- This part uses our NdeI/SacI/BamHI fusion cloning substandard
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]