Difference between revisions of "Part:BBa K3264022:Experience"

 
(Pathway for BBa_K1131000)
 
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how you used this part and how it worked out.
 
how you used this part and how it worked out.
  
===Applications of BBa_K3264022===
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== Pathway for BBa_K1131000==
 
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Flaving Containing-Monooxygenase (FMO) is an enzyme that can be used to produce indigo. The enzyme is involved in the conversion of indole to indoxyl using NADPH as its co-factor. And upon exposure to oxygen, indoxyl spontaneously dimerzes to indigo. Specifically, this is the pathway being employed: <br> <br>
===User Reviews===
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[[File:HBHB.png|cebter|200px]]
<!-- DON'T DELETE --><partinfo>BBa_K3264022 StartReviews</partinfo>
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<!--Upon cloning this enzyme into E. coli with the [https://parts.igem.org/Part:BBa_J23100 BBa_J23100 Anderson Promoter], and adding tryptophan (a precursor to indole) in the LB media, we observed a dense blue color in our cultures. <br>[[File:blue tube.jpg | 350px]]-->
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{|width='80%' style='border:1px solid gray'
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<partinfo>BBa_K3264022 AddReview number</partinfo>
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<I>Username</I>
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Enter the review inofrmation here.
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|};
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<!-- DON'T DELETE --><partinfo>BBa_K3264022 EndReviews</partinfo>
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Latest revision as of 15:26, 16 October 2020


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Please enter how you used this part and how it worked out.

Pathway for BBa_K1131000

Flaving Containing-Monooxygenase (FMO) is an enzyme that can be used to produce indigo. The enzyme is involved in the conversion of indole to indoxyl using NADPH as its co-factor. And upon exposure to oxygen, indoxyl spontaneously dimerzes to indigo. Specifically, this is the pathway being employed:

cebter