Difference between revisions of "Part:BBa K3408011"

Revision as of 14:33, 16 October 2020

P_liaG-B0034-lacI-P_grac-B0034-CⅠ-P_CⅠ-GFP-B0015

This composite part consists of promoter P_liaG and P_grac, lacI gene, CⅠ gene, GFP gene and some essential RBS and terminators. Promoter P_liaG starts transcription process and its transcription production is LacI, which is regulated by IPTG. When bacteria is exposed to medium which is rich in IPTG, bacteria can intake IPTG. IPTG is combined with LacI, then LacI cannot be bound to promoter Pgrac which is specially suppressed by LacI. Therefore, CⅠ repressor protein cannot generate as downstream cⅠ gene cannot transcribe regularly.

As a result, P_CⅠ recovers its activity and triggers the downstream transcription of GFP. By checking the expression of GFP, we could verify the feasibility of IPTG induction system, thus guaranteeing the successful culture of our engineered Bacillus subtilis.

Plasmid profile T--NAU-CHINA--composite-parts6.1.png

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1294
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 2155

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	This composite part consists of promoter P<sub>liaG</sub> and P<sub>grac</sub>, lacI gene, CⅠ gene, GFP gene and some essential RBS and terminators. Promoter P<sub>liaG</sub> starts transcription process and its transcription production is LacI, which is regulated by IPTG. When bacteria is exposed to medium which is rich in IPTG, bacteria can intake IPTG. IPTG is combined with LacI, then LacI cannot be bound to promoter Pgrac which is specially suppressed by LacI. Therefore, CⅠ repressor protein cannot generate as downstream cⅠ gene cannot transcribe regularly.		This composite part consists of promoter P<sub>liaG</sub> and P<sub>grac</sub>, lacI gene, CⅠ gene, GFP gene and some essential RBS and terminators. Promoter P<sub>liaG</sub> starts transcription process and its transcription production is LacI, which is regulated by IPTG. When bacteria is exposed to medium which is rich in IPTG, bacteria can intake IPTG. IPTG is combined with LacI, then LacI cannot be bound to promoter Pgrac which is specially suppressed by LacI. Therefore, CⅠ repressor protein cannot generate as downstream cⅠ gene cannot transcribe regularly.
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	As a result, P<sub>CⅠ</sub> recovers its activity and triggers the downstream transcription of GFP. By checking the expression of GFP, we could verify the feasibility of IPTG induction system, thus guaranteeing the successful culture of our engineered <i>Bacillus subtilis</i>.		As a result, P<sub>CⅠ</sub> recovers its activity and triggers the downstream transcription of GFP. By checking the expression of GFP, we could verify the feasibility of IPTG induction system, thus guaranteeing the successful culture of our engineered <i>Bacillus subtilis</i>.