Difference between revisions of "Part:BBa K3408010"

Revision as of 14:19, 16 October 2020

P_nar-B0034-Trigger RNA-B0015-P_CⅠ-Switch RNA-mazF-B0015

We designed this composite part by combining promoter P_nar, trigger RNA, P_CⅠ, switch RNA and mazF. So we could utilize it to verify the feasibility of our device, which comprises of Toehold switch and suicide protein—mazF. Former composite part BBa_K3408006 has demonstrated the feasibility of promoter P_nar, so when our engineered bacteria Bacillus subtilis is in anaerobic environment, promoter P_nar is activated by global regulator—FNR. Then downstream trigger RNA is expressed. As switch RNA is constantly expressed under the control of promoter P_C1, the trigger RNA can successfully trigger the Toehold switch, thus leading to the expression of mazF. So Bacillus subtilis can commit suicide. While in aerobic environment, promoter P_nar ceases to work, Toehold switch cannot be triggered. As a result, mazF can not express. This device proves that our suicide module and Toehold switch can successfully combine together.

Plasmid profile T--NAU-CHINA--composite-parts5.1.png

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 187
Illegal BglII site found at 409
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K3408010 short</partinfo>
-We designed this composite part by combining promoter P<sub>nar</sub>, trigger RNA, P<sub>CⅠ</sub>, switch RNA and mazF. So we could utilize it to verify the feasibility of our device, which comprises of Toehold switch and suicide protein—mazF. Former composite part BBa_K3408006 has demonstrated the feasibility of promoter P<sub>nar</sub>, so when our engineered bacteria <i>Bacillus subtilis</i> is in anaerobic environment, promoter P<sub>nar</sub> is activated by global regulator—FNR. Then downstream trigger RNA is expressed. As switch RNA is constantly expressed under the control of promoter P<sub>C1</sub>, the trigger RNA can successfully trigger the Toehold switch, thus leading to the expression of mazF. So <i>Bacillus subtilis</i> can commit suicide. While in aerobic environment, promoter P<sub>nar</sub> ceases to work, Toehold switch cannot be triggered. As a result, mazF can not express. This device proves that our suicide module and Toehold switch can successfully combine together.
+We designed this composite part by combining promoter P<sub>nar</sub>, trigger RNA, P<sub>CⅠ</sub>, switch RNA and mazF. So we could utilize it to verify the feasibility of our device, which comprises of Toehold switch and suicide protein—mazF.
+Former composite part BBa_K3408006 has demonstrated the feasibility of promoter P<sub>nar</sub>, so when our engineered bacteria <i>Bacillus subtilis</i> is in anaerobic environment, promoter P<sub>nar</sub> is activated by global regulator—FNR. Then downstream trigger RNA is expressed. As switch RNA is constantly expressed under the control of promoter P<sub>C1</sub>, the trigger RNA can successfully trigger the Toehold switch, thus leading to the expression of mazF. So <i>Bacillus subtilis</i> can commit suicide. While in aerobic environment, promoter P<sub>nar</sub> ceases to work, Toehold switch cannot be triggered. As a result, mazF can not express. This device proves that our suicide module and Toehold switch can successfully combine together.
 Plasmid profile