Difference between revisions of "Part:BBa K3408007"

Revision as of 13:44, 16 October 2020

P_nar-B0034-phy(ycD)-B0015

Used Pnar(BBa_K3408000), RBS(BBa_B0034), phy(ycD)(BBa_E0040) and terminator(BBa_B0015) to express Phy(ycD) under the control of P_nar. When our Bacillus subtilis was in an anerobic environment, Pnar was activated by FNR so that Phy(ycD) could be expressed. But when our Bacillus subtilis was in an aerobic environment, P_nar could be suppressed and we couldn’t detect more Phy(ycD).

Plasmid profile T--NAU-CHINA--composite-parts1.1.png

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 454
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3408007 short</partinfo>
-Used P<sub>nar</sub>(BBa_K3408000), RBS(BBa_B0034), optimized CⅠ(BBa_K3408004), terminator(BBa_B0015), P<sub>CⅠ</sub>(BBa_R0051), RBS(BBa_B0034) and GFP(BBa_E0040) to express GFP when PCⅠ was activated. When our <i>Bacillus subtilis</i> was in an aerobic environment, P<sub>nar</sub> was suppressed without FNR and no CⅠ protein could be expressed so that P<sub>CⅠ</sub> could be activated and we could see GFP expressed. But when our <i>Bacillus subtilis</i> was in an anerobic environment, CⅠ protein could be expressed to suppress P<sub>CⅠ</sub> and we couldn't see GFP anymore.
+Used Pnar(BBa_K3408000), RBS(BBa_B0034), phy(ycD)(BBa_E0040) and terminator(BBa_B0015) to express Phy(ycD) under the control of P<sub>nar</sub>. When our Bacillus subtilis was in an anerobic environment, Pnar was activated by FNR so that Phy(ycD) could be expressed. But when our <i>Bacillus subtilis</i> was in an aerobic environment, P<sub>nar</sub> could be suppressed and we couldn’t detect more Phy(ycD).
+Plasmid profile
+https://2020.igem.org/wiki/images/1/18/T--NAU-CHINA--composite-parts1.1.png
 <!-- Add more about the biology of this part here