Difference between revisions of "Part:BBa K3408006"

Revision as of 13:39, 16 October 2020

We used promoter P_nar which was activated by FNR to trigger the expression of GFP. If there was no oxygen, then FNR could be transcribed and we could see green bacteria in our culture medium.

Plasmid profile T--NAU-CHINA--composite-parts1.1.png

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-Used Pnar(BBa_K3408000), RBS(BBa_B0034), GFP(BBa_E0040) and terminator(BBa_B0015) to express GFP under the control of P<sub>nar</sub>. When our Bacillus subtilis was in an anerobic environment, Pnar was activated by FNR so that GFP could be expressed. But when our <i>Bacillus subtilis</i> was in an aerobic environment, P<sub>nar</sub> could be suppressed and we couldn't see GFP anymore.
+We used promoter P<sub>nar</sub> which was activated by FNR to trigger the expression of GFP. If there was no oxygen, then FNR could be transcribed and we could see green bacteria in our culture medium.
 Plasmid profile
 https://2020.igem.org/wiki/images/1/18/T--NAU-CHINA--composite-parts1.1.png