Difference between revisions of "Part:BBa K103009"

Latest revision as of 12:42, 28 October 2008

linker fused to alpha fragment of TEM beta-lactamase

alpha fragment of the split enzyme TEM β-lactamase
β-lactamase activity can be recovered when halves are brought in close proximity by e.g. fusion to interacting domains [http://www.ncbi.nlm.nih.gov/pubmed/17005637?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum]
works with second half of β-lactamase (part BBa_K103013)
contains linker module that allows proper binding of fusion partners
This part uses our NdeI/SacI/BamHI fusion cloning substandard

This protein structure was predicted using threading and protein fold prediction and may differ from actual one.

Sequence and Features

Assembly Compatibility:

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K103009 short</partinfo>
+===Usage and Biology===
+<!-- Add more about the biology of this part here -->
+{|
+|
+* alpha fragment of the split enzyme TEM &beta;-lactamase
+* &beta;-lactamase activity can be recovered when halves are brought in close proximity by e.g. fusion to interacting domains [http://www.ncbi.nlm.nih.gov/pubmed/17005637?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum]
-<!-- Add more about the biology of this part here -->
+* works with second half of &beta;-lactamase (part [https://parts.igem.org/Part:BBa_K103013 BBa_K103013])
-===Usage and Biology===
-* alpha fragment of the split enzyme TEM beta-lactamase
-* beta lactamase activity can be recovered when halves are brought in close proximity by e.g. fusion to interacting domains [http://www.ncbi.nlm.nih.gov/pubmed/17005637?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum]
-* works with second half of beta lactamase (part BBa_K103013)
 * contains linker module that allows proper binding of fusion partners
 * This part uses our NdeI/SacI/BamHI fusion cloning substandard
+| [[Image:Linkeralpha.jpg]]
+This protein structure was predicted using threading and protein fold prediction and may differ from actual one.
+|}