Difference between revisions of "Part:BBa K3409003:Design"

 
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Initially identified in cattle E. coli Strain SSuT-25 plasmid in 2009.
 
Initially identified in cattle E. coli Strain SSuT-25 plasmid in 2009.
−
Entire gene cluster found on GenBank (KJ563250.1): https://www.ncbi.nlm.nih.gov/nuccore/700369833  
+
Entire gene cluster found on GenBank (KJ563250.1): https://www.ncbi.nlm.nih.gov/nuccore/700369833 . Specific sequence for mcpM found on Genbank (AIU94005.1): https://www.ncbi.nlm.nih.gov/protein/700369842 .
−
Specific sequence for mcpM found on Genbank (AIU94005.1): https://www.ncbi.nlm.nih.gov/protein/700369842  
+
  
 
===References===
 
===References===

Revision as of 12:41, 16 October 2020


mcpM + His-tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Did not contain any illegal restriction sites, no codon optimization was necessary.


Source

Initially identified in cattle E. coli Strain SSuT-25 plasmid in 2009. Entire gene cluster found on GenBank (KJ563250.1): https://www.ncbi.nlm.nih.gov/nuccore/700369833 . Specific sequence for mcpM found on Genbank (AIU94005.1): https://www.ncbi.nlm.nih.gov/protein/700369842 .

References