Difference between revisions of "Part:BBa K3402000"
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===Contribution===
 
===Contribution===
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We optimized the codon sequence of yeGFP at the basic of [https://parts.igem.org/Part:BBa_E0040# BBa_E0040]to express it in <i>Starmerella bombicola</i> to characterize the strength of promoters.
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Because <i>Starmerella bombicola</i> is eukaryote, origin GFP gene can not express in it. So we optimized the codon sequence of <i>yeGFP</i> at the basic of [https://parts.igem.org/Part:BBa_E0040# BBa_E0040]to express it in <i>Starmerella bombicola</i> to characterize the strength of promoters.
  
 
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Revision as of 12:19, 16 October 2020


yeGFP

The yeGFP can express green fluorescence protein, which can be used as a report gene to characterize the strength of promoters.

Usage and Biology

We link different promoters to yeGFP to express green fluorescent protein, so we will know the strength of promoters by testing different fluorescence intensity of different promoters

Expression of yeGFP

Contribution

Because Starmerella bombicola is eukaryote, origin GFP gene can not express in it. So we optimized the codon sequence of yeGFP at the basic of BBa_E0040to express it in Starmerella bombicola to characterize the strength of promoters.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 13