Difference between revisions of "Part:BBa K3598000"

 
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<partinfo>BBa_K3598000 short</partinfo>
 
<partinfo>BBa_K3598000 short</partinfo>
  
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We added AOX1 promoter and terminator into a plasmid in Pichia pastoris containing the sequence of sfGFP and recorded the intensity of the expression of fluorescence proteins in our experiment. We added 5% methanol into the culture for 5 days, and extracted the supernatant and ran 4%-20% SDS-PAGE gel for every 24 hours.
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AOX1 is a very common inducible promoter in yeast, and its ON/OFF condition is controlled by methanol.
  
 
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Revision as of 07:51, 16 October 2020


AOX1 promoter

AOX1 is a very common inducible promoter in yeast, and its ON/OFF condition is controlled by methanol.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 937
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]