Difference between revisions of "Part:BBa K3570017"

 
 
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<partinfo>BBa_K3570017 short</partinfo>
 
<partinfo>BBa_K3570017 short</partinfo>
  
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YPRCtau3 downstream homologous arm used together with YPRCtau3 upstream (BBa_K3570016). When YPRCtau3 upstream put to 5' of the biobrick together with YPRCtau3 downstream to the 3', the biobrick can be integrated into the S. cerevisiae's genome. It will do a homologous recombination within a region of type 4 long terminal repeat (Ty4 LTR) in chromosome XVI.  
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<h2>Usage</h2>
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<p style="text-indent: 40px">
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YPRCtau3 downstream homology arm part shall be used together with YPRCtau3 upstream homology arm part ([https://parts.igem.org/Part:BBa_K3570016 BBa_K3570016]) to target a functional yeast integration locus. When YPRCtau3 upstream put to 5' of the biobrick together with YPRCtau3 downstream to the 3', the biobrick can be integrated into the <i>S. cerevisiae</i>'s genome. It will do a homologous recombination within a region of type 4 long terminal repeat (Ty4 LTR) in chromosome XVI. </p>
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<p style="text-indent: 40px">
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This sequence was identified from a personal communication with Dr. Jean-Marie Francois. </p>
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<h2>Experiments</h2>
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Team iGEM Toulouse 2020 did not have sufficient time to complete the cloning and hence, to test this part functionality.
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<h2>References</h2>
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<i>S. cerevisiae</i> genome, chromosome XVI, Ty4 LTR region. GenBank: CP046096.1
  
 
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Latest revision as of 14:42, 15 October 2020


YPRCtau3 downstream homologous sequence

Usage

YPRCtau3 downstream homology arm part shall be used together with YPRCtau3 upstream homology arm part (BBa_K3570016) to target a functional yeast integration locus. When YPRCtau3 upstream put to 5' of the biobrick together with YPRCtau3 downstream to the 3', the biobrick can be integrated into the S. cerevisiae's genome. It will do a homologous recombination within a region of type 4 long terminal repeat (Ty4 LTR) in chromosome XVI.

This sequence was identified from a personal communication with Dr. Jean-Marie Francois.

Experiments

Team iGEM Toulouse 2020 did not have sufficient time to complete the cloning and hence, to test this part functionality.

References

S. cerevisiae genome, chromosome XVI, Ty4 LTR region. GenBank: CP046096.1

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 625
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]