Difference between revisions of "Part:BBa K3570014"

Latest revision as of 14:34, 15 October 2020

YPRCdelta15 upstream homologous sequence

Usage

YPRCdelta15 upstream homology arm part shall be used together with YPRCdelta15 downstream homology arm part (BBa_K3570015) to target a functional yeast integration locus. When YPRCdelta15 upstream put to 5' of the biobrick together with YPRCdelta15 downstream to the 3', the biobrick can be integrated into the S. cerevisiae's genome. It will do a homologous recombination within a region of type 1 long terminal repeat (Ty1 LTR) in chromosome XVI.

Experiments

Team iGEM Toulouse 2020 did not have sufficient time to complete the cloning and hence, to test this part functionality.

References

Bai Flagfeldt, D., Siewers, V., Huang, L., & Nielsen, J. (2009). Characterization of chromosomal integration sites for heterologous gene expression inSaccharomyces cerevisiae. Yeast, 26(10), 545–551. https://doi.org/10.1002/yea.1705
S. cerevisiae genome, chromosome XVI, Ty1 LTR region. GenBank: CP046096.1

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 10: / Line 10: @@
 Team iGEM Toulouse 2020 did not have sufficient time to complete the cloning and hence, to test this part functionality.
 <h2>References</h2>
-<i>S. cerevisiae</i> genome, chromosome XVI, Ty1 LTR region. GenBank: CP046096.1
+*Bai Flagfeldt, D., Siewers, V., Huang, L., & Nielsen, J. (2009). Characterization of chromosomal integration sites for heterologous gene expression inSaccharomyces cerevisiae. Yeast, 26(10), 545–551. https://doi.org/10.1002/yea.1705
+*<i>S. cerevisiae</i> genome, chromosome XVI, Ty1 LTR region. GenBank: CP046096.1