Difference between revisions of "Part:BBa K3570010"

 
 
(6 intermediate revisions by the same user not shown)
Line 3: Line 3:
 
<partinfo>BBa_K3570010 short</partinfo>
 
<partinfo>BBa_K3570010 short</partinfo>
  
−
HO downstream homologous arm used together with HO upstream (BBa_K3570009). When HO upstream put to 5' of the biobrick together with HO downstream to the 3', the biobrick can be integrated into the S. cerevisiae's genome. It will do a homologous recombination within the Homothallic switching endonuclease (HO) gene.
+
<h2>Usage</h2>
 +
<p style="text-indent: 40px">
 +
HO downstream homology arm part used together with HO upstream homology arm part ([https://parts.igem.org/Part:BBa_K3570009 BBa_K3570009]) to target a functional yeast integration locus. When HO upstream put to 5' of the biobrick together with HO downstream to the 3', the biobrick can be integrated into the <i>S. cerevisiae</i>'s genome. It will do homologous recombination within the Homothallic switching endonuclease (HO) gene.</p>
 +
<p style="text-indent: 40px">
 +
This sequence was identified from a personal communication with Dr. Gilles Truan. </p>
 +
 
 +
<h2>Experiments</h2>
 +
Team iGEM Toulouse 2020 did not have sufficient time to complete the cloning and hence, to test this part functionality.
 +
 
 +
<h2>References</h2>
 +
 
 +
*[1]<i>S. cerevisiae</i> genome, chromosome IV, HO gene. GenBank: CP046084.1
 +
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 14:03, 15 October 2020


HO downstream homologous sequence

Usage

HO downstream homology arm part used together with HO upstream homology arm part (BBa_K3570009) to target a functional yeast integration locus. When HO upstream put to 5' of the biobrick together with HO downstream to the 3', the biobrick can be integrated into the S. cerevisiae's genome. It will do homologous recombination within the Homothallic switching endonuclease (HO) gene.

This sequence was identified from a personal communication with Dr. Gilles Truan.

Experiments

Team iGEM Toulouse 2020 did not have sufficient time to complete the cloning and hence, to test this part functionality.

References

  • [1]S. cerevisiae genome, chromosome IV, HO gene. GenBank: CP046084.1


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]