Difference between revisions of "Part:BBa K3360003:Design"
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Revision as of 13:43, 15 October 2020
IARI-SP-2
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1587
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Encoding the endo-beta-1,4-glucanase of Bacillus subtilis, the entire sequence has been optimized by E.coli codons, and the N-terminus is marked with GST to increase its solubility and reduce the formation of inclusion bodies.
Source
Encoding the endo-beta-1,4-glucanase of Bacillus subtilis, the entire sequence has been optimized by E.coli codons, and the N-terminus is marked with GST to increase its solubility and reduce the formation of inclusion bodies.