Difference between revisions of "Part:BBa K079017"
 
(19 intermediate revisions by the same user not shown)
Line 4: Line 4:
  
 
This parts have been extracted from the [https://parts.igem.org/wiki/index.php?title=Part:BBa_K079045 Lac Operator Library].
 
This parts have been extracted from the [https://parts.igem.org/wiki/index.php?title=Part:BBa_K079045 Lac Operator Library].
−
<br><br><br>
+
<br><br>
 +
[[Image:LacOperon.jpg |left|350px|thumbnail|Regulatory Elements position in natural Lac Operon ]]
 
[https://parts.igem.org/wiki/index.php?title=Part:BBa_K079018 K079018]: Lac O1 is the strongest wild type operator sequence in Lac Operon. In the natural system is located right after the -10 consensus sequence.
 
[https://parts.igem.org/wiki/index.php?title=Part:BBa_K079018 K079018]: Lac O1 is the strongest wild type operator sequence in Lac Operon. In the natural system is located right after the -10 consensus sequence.
  
 
[https://parts.igem.org/wiki/index.php?title=Part:BBa_K079019 K079019]: LacO2 is the intermediate affinity operator site, located 400 bp downstream of O1. In natural Lac Operon is responsible for Dna Looping, enanching repression and cooperativity of Lac system.
 
[https://parts.igem.org/wiki/index.php?title=Part:BBa_K079019 K079019]: LacO2 is the intermediate affinity operator site, located 400 bp downstream of O1. In natural Lac Operon is responsible for Dna Looping, enanching repression and cooperativity of Lac system.
  
−
[https://parts.igem.org/wiki/index.php?title=Part:BBa_K079017 K079017]: SymL is a perfectly symmetric Lac operator synthetically designed to maximize operator-repressor interaction. Its binding is about '''10-fold''' tighter than the O1 natural sequence
+
[https://parts.igem.org/wiki/index.php?title=Part:BBa_K079017 K079017]: SymL or Oid is a perfectly symmetric Lac operator synthetically designed to maximize operator-repressor interaction. Its binding is about '''10-fold''' tighter than the O1 natural sequence
 +
 
 +
<br><br><br>
 +
 
 +
 
 +
[[Image:LacAffinity.jpg |right|400px|thumbnail|Single Lac Operators Repression Strength defined as Unepressed versus Repressed LacZ Activity ratio ]]
 +
This subset of operator sequences has been chosen since it allows to cover a wide affinity and repression range
  
−
[[Image:LacOperon.jpg |right|300px ]]
+
<br>
 +
The parts can be individually assembled with [https://parts.igem.org/wiki/index.php?title=Part:BBa_J23103 Constitutive Promoter Family] or arranged to obtain a variety of Lac regulated promoters.
 +
<br>
 +
Combinations of different operator types and distances should give rise to promoter elements with different repression strength and cooperativiy
  
  
 +
<br><br><br><br><br><br>
 
<br><br><br><br><br><br>
 
<br><br><br><br><br><br>
  

Latest revision as of 12:29, 28 October 2008

Lac symmetric - operator library member

This parts have been extracted from the Lac Operator Library.

Regulatory Elements position in natural Lac Operon

K079018: Lac O1 is the strongest wild type operator sequence in Lac Operon. In the natural system is located right after the -10 consensus sequence.

K079019: LacO2 is the intermediate affinity operator site, located 400 bp downstream of O1. In natural Lac Operon is responsible for Dna Looping, enanching repression and cooperativity of Lac system.

K079017: SymL or Oid is a perfectly symmetric Lac operator synthetically designed to maximize operator-repressor interaction. Its binding is about 10-fold tighter than the O1 natural sequence





Single Lac Operators Repression Strength defined as Unepressed versus Repressed LacZ Activity ratio

This subset of operator sequences has been chosen since it allows to cover a wide affinity and repression range


The parts can be individually assembled with Constitutive Promoter Family or arranged to obtain a variety of Lac regulated promoters.
Combinations of different operator types and distances should give rise to promoter elements with different repression strength and cooperativiy















Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]