Difference between revisions of "Part:BBa K103013"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | * | + | * omega fragment of the split enzyme TEM β-lactamase |
| − | * beta-lactamase activity can be recovered when halves are brought in close proximity by e.g. fusion to interacting domains [http://www.ncbi.nlm.nih.gov/pubmed/17005637?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum] | + | * β-lactamase activity can be recovered when halves are brought in close proximity by e.g. fusion to interacting domains [http://www.ncbi.nlm.nih.gov/pubmed/17005637?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum] |
| − | * works with second half of beta lactamase (part BBa_K103009) | + | * works with second half of β-lactamase (part [https://parts.igem.org/Part:BBa_K103009 BBa_K103009]) |
* contains linker module that allows proper binding of fusion partners | * contains linker module that allows proper binding of fusion partners | ||
* This part uses our NdeI/SacI/BamHI fusion cloning substandard | * This part uses our NdeI/SacI/BamHI fusion cloning substandard | ||
| − | |[[Image:Linkermoega.jpg]] | + | | [[Image:Linkermoega.jpg]] |
| + | This protein structure was predicted using threading and protein fold prediction and may differ from actual one. | ||
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Latest revision as of 12:28, 28 October 2008
linker fused to omega fragment of TEM beta-lactamase
Usage and Biology
|
This protein structure was predicted using threading and protein fold prediction and may differ from actual one. |
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 190