Difference between revisions of "Part:BBa K115002:Design"
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===Design Notes=== | ===Design Notes=== | ||
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| + | The figure shows the secondary structure of the original RNA thermometer as well as the secondary structure of this part. Because of the scar that is introduced during the ligation process, some nucleotides had to be changed in order to get the same secondary structure as that of the original one. The changed nucleotides are the ones with the blue dots. | ||
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| + | The working of the RNA thermometer is based on the secondary structure and that is why it is important that this part has the same secondary structure after ligation to a protein coding part. | ||
[[Image:BBa_K115002.png | 600px]] | [[Image:BBa_K115002.png | 600px]] | ||
Revision as of 12:24, 28 October 2008
RNA thermometer (FourU)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The figure shows the secondary structure of the original RNA thermometer as well as the secondary structure of this part. Because of the scar that is introduced during the ligation process, some nucleotides had to be changed in order to get the same secondary structure as that of the original one. The changed nucleotides are the ones with the blue dots.
The working of the RNA thermometer is based on the secondary structure and that is why it is important that this part has the same secondary structure after ligation to a protein coding part.
Source
Salmonella Enterica Tyhpy (CP000886.1)