Difference between revisions of "Part:BBa K079017"

Revision as of 12:11, 28 October 2008

Lac symmetric - operator library member

This parts have been extracted from the Lac Operator Library.

Regulatory Elements position in natural Lac Operon

K079018: Lac O1 is the strongest wild type operator sequence in Lac Operon. In the natural system is located right after the -10 consensus sequence.

K079019: LacO2 is the intermediate affinity operator site, located 400 bp downstream of O1. In natural Lac Operon is responsible for Dna Looping, enanching repression and cooperativity of Lac system.

K079017: SymL or Oid is a perfectly symmetric Lac operator synthetically designed to maximize operator-repressor interaction. Its binding is about 10-fold tighter than the O1 natural sequence

Single Lac Operators Repression Strength defined as Repressed-Unrepressed LacZ Activity ratio

This subset of operator sequences has been chosen since it allows to cover a wide affinity and repression range

Sequence and Features