Difference between revisions of "Part:BBa K3425033:Design"

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__NOTOC__
 
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<partinfo>BBa_K3425025 short</partinfo>
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<partinfo>BBa_K3425033 short</partinfo>
  
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<partinfo>BBa_K3425025 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K3425033 SequenceAndFeatures</partinfo>
  
  
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===Source===
 
===Source===
  
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iGEM Registry of Standard Parts BBa_B0030
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iGEM Registry of Standard Biological Parts BBa_B0030
  
 
===References===
 
===References===

Revision as of 10:27, 14 October 2020


RBS for Type IIS iGEM Standard Assembly


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The spacer provides aligned spacing (the distance between RBS and ATG) of 10 nucleotides when Type IIS iGEM Standard assembly is used.

Source

iGEM Registry of Standard Biological Parts BBa_B0030

References

For aligned spacing refer:

Ma, J., Campbell, A., and Karlin, S. (2002) Correlations between Shine-Dalgarno Sequences and Gene Features Such as Predicted Expression Levels and Operon Structures. J. Bacteriol. 184, 5733–5745