Difference between revisions of "Part:BBa K3570007"
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<h2>Usage</h2> | <h2>Usage</h2> | ||
<p style="text-indent: 40px"> | <p style="text-indent: 40px"> | ||
− | DPP1 downstream homology arm part shall be used together with DPP1 downstream homology arm part ([https://parts.igem.org/Part:BBa_K3570006 BBa_K3570006]) to | + | DPP1 downstream homology arm part shall be used together with DPP1 downstream homology arm part ([https://parts.igem.org/Part:BBa_K3570006 BBa_K3570006]) to target a functional yeast integration locus. When DPP1 upstream put to 5' of the biobrick together with DPP1 downstream to the 3', the biobrick can be integrated into the <i>S. cerevisiae</i>'s genome. It will do homologous recombination within the Diacylglycerol pyrophosphate phosphatase 1 (DPP1) gene.</p> |
<h2>Experiments</h2> | <h2>Experiments</h2> |
Revision as of 04:48, 14 October 2020
DPP1 downstream homologous sequence
Usage
DPP1 downstream homology arm part shall be used together with DPP1 downstream homology arm part (BBa_K3570006) to target a functional yeast integration locus. When DPP1 upstream put to 5' of the biobrick together with DPP1 downstream to the 3', the biobrick can be integrated into the S. cerevisiae's genome. It will do homologous recombination within the Diacylglycerol pyrophosphate phosphatase 1 (DPP1) gene.
Experiments
Referencesss
S. cerevisiae genome, chromosome IV, DPP1 gene. GenBank: CP046084.1
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]