Difference between revisions of "Part:BBa K104001:Design"
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A terminator was included after the spaRK to prevent read through. The native SpaRK promoter is controlled by the sigma factor sigma-H and induced at the end of exponential phase. The regulation by sigma-H was left unmodified. | A terminator was included after the spaRK to prevent read through. The native SpaRK promoter is controlled by the sigma factor sigma-H and induced at the end of exponential phase. The regulation by sigma-H was left unmodified. | ||
| − | This | + | This Part was built from virtual parts, computationally modeled and then synthesised in its entirety by GenScript. |
===Source=== | ===Source=== | ||
Revision as of 11:33, 28 October 2008
Sensor for small peptide Subtilin
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
A terminator was included after the spaRK to prevent read through. The native SpaRK promoter is controlled by the sigma factor sigma-H and induced at the end of exponential phase. The regulation by sigma-H was left unmodified.
This Part was built from virtual parts, computationally modeled and then synthesised in its entirety by GenScript.
Source
The two component system, its promoter and RBS's, and the pSpaS promoter sequence are all derived from the genome of Bacillus subtilis ATCC 6633.
References
C Klein, C Kaletta, N Schnell and K D Entian (1992). Analysis of genes involved in biosynthesis of the lantibiotic subtilin. Appl Environ Microbiol. 58(1): 132-142.
C Klein, C Kaletta and K D Entian (1993). Biosynthesis of the lantibiotic subtilin is regulated by a histidine kinase/response regulator system. Appl Environ Microbiol. 59(1): 296-303.