Difference between revisions of "Part:BBa K079017"

Revision as of 11:32, 28 October 2008

Lac symmetric - operator library member

This parts have been extracted from the Lac Operator Library. K079018: Lac O1 is the strongest wild type operator sequence in Lac Operon. In the natural system is located right after the -10 consensus sequence.

K079019: LacO2 is the intermediate affinity operator site, located 400 bp downstream of O1. In natural Lac Operon is responsible for Dna Looping, enanching repression and cooperativity of Lac system.

K079017: SymL is a perfectly symmetric Lac operator synthetically designed to maximize operator-repressor interaction. Its binding is about 10-fold tighter than the O1 natural sequence

[Operon |500px]

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <br>
-This parts have been extracted from the [https://parts.igem.org/wiki/index.php?title=Part:BBa_K079045 Lac Operator Library]
+This parts have been extracted from the [https://parts.igem.org/wiki/index.php?title=Part:BBa_K079045 Lac Operator Library].
+[https://parts.igem.org/wiki/index.php?title=Part:BBa_K079018 K079018]: Lac O1 is the strongest wild type operator sequence in Lac Operon. In the natural system is located right after the -10 consensus sequence.
+[https://parts.igem.org/wiki/index.php?title=Part:BBa_K079019 K079019]: LacO2 is the intermediate affinity operator site, located 400 bp downstream of O1. In natural Lac Operon is responsible for Dna Looping, enanching repression and cooperativity of Lac system.
+[https://parts.igem.org/wiki/index.php?title=Part:BBa_K079017 K079017]: SymL is a perfectly symmetric Lac operator synthetically designed to maximize operator-repressor interaction. Its binding is about '''10-fold''' tighter than the O1 natural sequence
+[Operon |500px]
 <!-- Add more about the biology of this part here