Difference between revisions of "Part:BBa K3505006:Design"
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===Source=== | ===Source=== | ||
Latest revision as of 10:32, 13 October 2020
Tyr1-AIDAc Tyrosinase fused to membrane protein AIDAc. GB compatible B3-B5
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1439
Illegal EcoRI site found at 2135
Illegal XbaI site found at 1043
Illegal PstI site found at 1487
Illegal PstI site found at 2073 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1439
Illegal EcoRI site found at 2135
Illegal PstI site found at 1487
Illegal PstI site found at 2073
Illegal NotI site found at 1211 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1439
Illegal EcoRI site found at 2135
Illegal BglII site found at 471
Illegal BamHI site found at 454
Illegal BamHI site found at 2337 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1439
Illegal EcoRI site found at 2135
Illegal XbaI site found at 1043
Illegal PstI site found at 1487
Illegal PstI site found at 2073 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1439
Illegal EcoRI site found at 2135
Illegal XbaI site found at 1043
Illegal PstI site found at 1487
Illegal PstI site found at 2073
Illegal AgeI site found at 533 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is present in pUPD2 as Level 0 and has overhangs compatible for Golden Braid cloning. The CDS has position B3-B5.
Figure 1. The GB2.0 grammar
Source
- Tyr1 from Rhizobium etli in the supplementary of [3]
- AIDA from E.coli in the supplementary of [2]
References
- [1] Eric VanArsdale, David Hörnström, Gustav Sjöberg, Ida Järbur, Juliana Pitzer, Gregory F. Payne, Antonius J. A. van Maris, and William E. Bentley. A Coculture Based Tyrosine-Tyrosinase Electrochemical Gene Circuit for Connecting Cellular Communication with Electronic Networks.ACS Synthetic Biology 2020 9 (5), 1117-1128
DOI: 10.1021/acssynbio.9b00469
- [2] Gustavsson, M., Hörnström, D., Lundh, S. et al. Biocatalysis on the surface of Escherichia coli: melanin pigmentation of the cell exterior. Sci Rep 6, 36117 (2016). https://doi.org/10.1038/srep36117
- [3] David Hörnström, Gen Larsson, Antonius J.A. van Maris, Martin Gustavsson, Molecular optimization of autotransporter-based tyrosinase surface display, Biochimica et Biophysica Acta (BBA) - Biomembranes , Volume 1861, Issue 2, 2019, Pages 486-494, https://doi.org/10.1016/j.bbamem.2018.11.012.