Difference between revisions of "Part:BBa K104001:Design"
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A terminator was included after the spaRK to prevent read through. The native SpaRK promoter is controlled by the sigma factor sigma-H and induced at the end of exponential phase. The regulation by sigma-H was left unmodified. | A terminator was included after the spaRK to prevent read through. The native SpaRK promoter is controlled by the sigma factor sigma-H and induced at the end of exponential phase. The regulation by sigma-H was left unmodified. | ||
| − | This part was computationally modeled and then synthesised by GenScript as a single | + | This part was built from virtual parts, computationally modeled and then synthesised by GenScript as a single BioBrick. |
===Source=== | ===Source=== | ||
Revision as of 11:14, 28 October 2008
Sensor for small peptide Subtilin
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
A terminator was included after the spaRK to prevent read through. The native SpaRK promoter is controlled by the sigma factor sigma-H and induced at the end of exponential phase. The regulation by sigma-H was left unmodified.
This part was built from virtual parts, computationally modeled and then synthesised by GenScript as a single BioBrick.
Source
The two component system, its promoter and RBS's, and the pSpaS promoter sequence are all derived from the genome of Bacillus subtilis ATCC 6633.
References
C Klein, C Kaletta, N Schnell and K D Entian (1992). Analysis of genes involved in biosynthesis of the lantibiotic subtilin. Appl Environ Microbiol. 58(1): 132-142.
C Klein, C Kaletta and K D Entian (1993). Biosynthesis of the lantibiotic subtilin is regulated by a histidine kinase/response regulator system. Appl Environ Microbiol. 59(1): 296-303.