Difference between revisions of "Part:BBa K3570008"

Revision as of 07:29, 13 October 2020

HIS3 selection marker

Usage

HIS3 gene, found in the Saccharomyces cerevisiae yeast, encodes a protein called Imidazoleglycerol-phosphate dehydratase which catalyzes the sixth step in histidine biosynthesis(1). It is analogous to hisB in Escherichia coli.

HIS3 gene serves as a commonly used yeast selectable marker. When HIS3 gene is inserted into an integrative or replicative plasmid, it selects and maintains a yeast vector in yeast host cells. Those cells should not have the functional HIS3 gene in its genome[1].

The sequence contains HIS3 specific promoter, HIS3 coding sequence, and HIS3 terminator.

Experiments

References

[1]- Old, R. W., & Primrose, S. B. (1981). Principles of gene manipulation: an introduction to genetic engineering (Vol. 2). Univ of California Press.
[2]- GenBank: U03439.1
[3]- RS313 plasmid
[4]- SGD:S000005728

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1005
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 896
Illegal BglII site found at 956
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 <h2>Usage</h2>
 <p style="text-indent: 40px">
-HIS3 gene, found in the <i>Saccharomyces cerevisiae</i> yeast, encodes a protein called Imidazoleglycerol-phosphate dehydratase which catalyzes the sixth step in histidine biosynthesis(1). It is analogous to hisB in Escherichia coli.
+HIS3 gene, found in the <i>Saccharomyces cerevisiae</i> yeast, encodes a protein called Imidazoleglycerol-phosphate dehydratase which catalyzes the sixth step in histidine biosynthesis(1). It is analogous to hisB in Escherichia coli.</p>
+<p style="text-indent: 40px">
 HIS3 gene serves as a commonly used yeast selectable marker. When HIS3 gene is inserted into an integrative or replicative plasmid, it selects and maintains a yeast vector in yeast host cells. Those cells should not have the functional HIS3 gene in its genome[1]. </p>
 <p style="text-indent: 40px">
@@ Line 14: / Line 15: @@
 <h2>References</h2>
-*[1]Old, R. W., & Primrose, S. B. (1981). Principles of gene manipulation: an introduction to genetic engineering (Vol. 2). Univ of California Press.
+*[1]- Old, R. W., & Primrose, S. B. (1981). Principles of gene manipulation: an introduction to genetic engineering (Vol. 2). Univ of California Press.
-*[2]GenBank: U03439.1
+*[2]- GenBank: U03439.1
-*[3]RS313 plasmid https://www.addgene.org/vector-database/3972/
+*[3]- [https://www.addgene.org/vector-database/3972/ RS313 plasmid]
-*[4]https://web.archive.org/web/20060505213339/http://db.yeastgenome.org/cgi-bin/locus.pl?locus=his3
+*[4]- [https://www.yeastgenome.org/locus/s000005728 SGD:S000005728]
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