Difference between revisions of "Part:BBa K3570018"
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<partinfo>BBa_K3570018 short</partinfo>
 
<partinfo>BBa_K3570018 short</partinfo>
  
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The MET17 gene, found in the <i>Saccharomyces cerevisiae</i> yeast, encodes a bifunctional enzyme with O-acetylserine and O-acetylhomoserine sulfhydrylase activities involved in methionine and cysteine metabolism. It catalyses the reaction between acetylated serine or homoserine with thiol to produce the corresponding amino acid and is localized to the cytoplasm and plasma membrane.  
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<h2>Usage</h2>
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<p style="text-indent: 40px">
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The MET17 gene, found in the <i>Saccharomyces cerevisiae</i> yeast, encodes a bifunctional enzyme with O-acetylserine and O-acetylhomoserine sulfhydrylase activities involved in methionine and cysteine metabolism. It catalyzes the reaction between acetylated serine or homoserine with thiol to produce the corresponding amino acid and is localized to the cytoplasm and plasma membrane. </p>
  
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When inserted into an integrative or replicative plasmid, MET17 allows to counter-select the cells that acquired the prototroph character for methionine.
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<p style="text-indent: 40px">
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When inserted into an integrative or replicative plasmid, MET17 allows to counter-select the cells that acquired the prototroph character for methionine.</p>
  
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References:
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<h2>Experiments</h2>
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Team iGEM Toulouse 2020 did not have sufficient time to complete the cloning and hence, to test this part functionality.
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<h2>References</h2>
 
*[1]- [https://www.yeastgenome.org/locus/S000004294 SGD:S000004294]
 
*[1]- [https://www.yeastgenome.org/locus/S000004294 SGD:S000004294]
  

Revision as of 07:16, 13 October 2020


MET17 selection marker

Usage

The MET17 gene, found in the Saccharomyces cerevisiae yeast, encodes a bifunctional enzyme with O-acetylserine and O-acetylhomoserine sulfhydrylase activities involved in methionine and cysteine metabolism. It catalyzes the reaction between acetylated serine or homoserine with thiol to produce the corresponding amino acid and is localized to the cytoplasm and plasma membrane.

When inserted into an integrative or replicative plasmid, MET17 allows to counter-select the cells that acquired the prototroph character for methionine.

Experiments

Team iGEM Toulouse 2020 did not have sufficient time to complete the cloning and hence, to test this part functionality.

References

  • [2]- Masselot, M., & de Robichon-Szulmajster, H. (1975). Methionine biosynthesis in Saccharomyces cerevisiae. Molecular and General Genetics MGG, 139(2), 121–132. https://doi.org/10.1007/bf00264692

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 649
    Illegal XbaI site found at 378
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 649
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 649
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 649
    Illegal XbaI site found at 378
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 649
    Illegal XbaI site found at 378
    Illegal NgoMIV site found at 268
  • 1000
    COMPATIBLE WITH RFC[1000]