Difference between revisions of "Part:BBa K3570016"

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<partinfo>BBa_K3570016 short</partinfo>
 
<partinfo>BBa_K3570016 short</partinfo>
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<h2>Usage</h2>
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<p style="text-indent: 40px">
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YPRCtau3 upstream homology arm part shall be used together with YPRCtau3 downstream homology arm part ([https://parts.igem.org/Part:BBa_K3570017 BBa_K3570017]). When YPRCtau3 upstream put to 5' of the biobrick together with YPRCtau3 downstream to the 3', the biobrick can be integrated into the S. cerevisiae's genome. It will do homologous recombination within a region of type 4 long terminal repeat (Ty4 LTR) in chromosome XVI.</p>
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<h2>Experiments</h2>
  
YPRCtau3 upstream homologous arm used together with YPRCtau3 downstream (BBa_K3570017). When YPRCtau3 upstream put to 5' of the biobrick together with YPRCtau3 downstream to the 3', the biobrick can be integrated into the S. cerevisiae's genome. It will do a homologous recombination within a region of type 4 long terminal repeat (Ty4 LTR) in chromosome XVI.
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<h2>References</h2>
 
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<!-- Add more about the biology of this part here
 
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===Usage and Biology===
 
===Usage and Biology===

Revision as of 06:25, 13 October 2020


YPRCtau3 upstream homologous sequence

Usage

YPRCtau3 upstream homology arm part shall be used together with YPRCtau3 downstream homology arm part (BBa_K3570017). When YPRCtau3 upstream put to 5' of the biobrick together with YPRCtau3 downstream to the 3', the biobrick can be integrated into the S. cerevisiae's genome. It will do homologous recombination within a region of type 4 long terminal repeat (Ty4 LTR) in chromosome XVI.

Experiments

References

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 137
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 137
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 524
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 137
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 137
  • 1000
    COMPATIBLE WITH RFC[1000]