Difference between revisions of "Part:BBa K3570008"
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<h2>Usage</h2>
 
<h2>Usage</h2>
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The HIS3 gene, found in the <i>Saccharomyces cerevisiae</i> yeast, encodes a protein called Imidazoleglycerol-phosphate dehydratase which catalyses the sixth step in histidine biosynthesis(1). It is analogous to hisB in Escherichia coli.
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HIS3 gene, found in the <i>Saccharomyces cerevisiae</i> yeast, encodes a protein called Imidazoleglycerol-phosphate dehydratase which catalyzes the sixth step in histidine biosynthesis(1). It is analogous to hisB in Escherichia coli.
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HIS3 gene serves as a commonly used yeast selectable marker. When HIS3 gene is inserted into an integrative or replicative plasmid, it selects and maintains a yeast vector in yeast host cells[1]. </p>
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<p style="text-indent: 40px">
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The sequence contains HIS3 specific promoter, HIS3 coding sequence, and HIS3 terminator.</p>
  
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When inserted into an integrative or replicative plasmid, HIS3 allows to counter-select the cells that acquired the wanted plasmid.
 
  
 
<h2>Source</h2>
 
<h2>Source</h2>
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*[1]Old, R. W., & Primrose, S. B. (1981). Principles of gene manipulation: an introduction to genetic engineering (Vol. 2). Univ of California Press.
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*[1]GenBank: U03439.1
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*[2]GenBank: U03439.1
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*[2]RS313 plasmid https://www.addgene.org/vector-database/3972/
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*[3]RS313 plasmid https://www.addgene.org/vector-database/3972/
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*[3]https://web.archive.org/web/20060505213339/http://db.yeastgenome.org/cgi-bin/locus.pl?locus=his3
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*[4]https://web.archive.org/web/20060505213339/http://db.yeastgenome.org/cgi-bin/locus.pl?locus=his3
  
 
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Revision as of 18:52, 12 October 2020


HIS3 selection marker

Usage

HIS3 gene, found in the Saccharomyces cerevisiae yeast, encodes a protein called Imidazoleglycerol-phosphate dehydratase which catalyzes the sixth step in histidine biosynthesis(1). It is analogous to hisB in Escherichia coli. HIS3 gene serves as a commonly used yeast selectable marker. When HIS3 gene is inserted into an integrative or replicative plasmid, it selects and maintains a yeast vector in yeast host cells[1].

The sequence contains HIS3 specific promoter, HIS3 coding sequence, and HIS3 terminator.


Source

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1005
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 896
    Illegal BglII site found at 956
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]