Difference between revisions of "Part:BBa K3570008"

Revision as of 18:39, 12 October 2020

HIS3 selection marker

Usage

The HIS3 gene, found in the Saccharomyces cerevisiae yeast, encodes a protein called Imidazoleglycerol-phosphate dehydratase which catalyses the sixth step in histidine biosynthesis(1). It is analogous to hisB in Escherichia coli.

When inserted into an integrative or replicative plasmid, HIS3 allows to counter-select the cells that acquired the wanted plasmid.

Source

[1]GenBank: U03439.1
[2]RS313 plasmid https://www.addgene.org/vector-database/3972/
[3]https://web.archive.org/web/20060505213339/http://db.yeastgenome.org/cgi-bin/locus.pl?locus=his3

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1005
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 896
Illegal BglII site found at 956
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3570008 short</partinfo>
+<h2>Usage</h2>
 The HIS3 gene, found in the <i>Saccharomyces cerevisiae</i> yeast, encodes a protein called Imidazoleglycerol-phosphate dehydratase which catalyses the sixth step in histidine biosynthesis(1). It is analogous to hisB in Escherichia coli.
 When inserted into an integrative or replicative plasmid, HIS3 allows to counter-select the cells that acquired the wanted plasmid.
-Sources
+<h2>Source</h2>
-- RS313 plasmid https://www.addgene.org/vector-database/3972/
-- https://web.archive.org/web/20060505213339/http://db.yeastgenome.org/cgi-bin/locus.pl?locus=his3
+*[1]GenBank: U03439.1
+*[2]RS313 plasmid https://www.addgene.org/vector-database/3972/
+*[3]https://web.archive.org/web/20060505213339/http://db.yeastgenome.org/cgi-bin/locus.pl?locus=his3
 <!-- Add more about the biology of this part here