Difference between revisions of "Part:BBa K3629020"
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===Usage and Biology=== | ===Usage and Biology=== | ||
+ | 2-isopropylmalate synthase is the first enzyme involved in the biosynthesis of leucine. This is a very important enzyme in the pathway as it serves as a regulatory point in the biosynthesis pathway. The protein has a leucine binding domain which allows for down regulation of the pathway through negative-feedback inhibition of the enzyme. Expression of this enzyme especially on a strong promoter could be used to upregulate leucine synthesis and produce a leucine overproducing strain. | ||
===Design=== | ===Design=== |
Revision as of 07:37, 12 October 2020
Yarrowia lipolytica TRP2 Gene
Arshia insert
Usage and Biology
2-isopropylmalate synthase is the first enzyme involved in the biosynthesis of leucine. This is a very important enzyme in the pathway as it serves as a regulatory point in the biosynthesis pathway. The protein has a leucine binding domain which allows for down regulation of the pathway through negative-feedback inhibition of the enzyme. Expression of this enzyme especially on a strong promoter could be used to upregulate leucine synthesis and produce a leucine overproducing strain.
Design
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 943
Illegal NgoMIV site found at 1213
Illegal NgoMIV site found at 1294
Illegal NgoMIV site found at 1393 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 283
Illegal BsaI.rc site found at 544
Illegal BsaI.rc site found at 1441