Difference between revisions of "Part:BBa K3408011"

 
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We used constitutive promoter P<sub>liaG</sub> to transcribe trigger RNA all the time. And we also used P<sub>C&#8544;</sub> as a constitutive promoter to transcribe switch RNA. If our Toehold switch could work normally, GFP could be translated and we could see green bacteria in our culture medium.
 
We used constitutive promoter P<sub>liaG</sub> to transcribe trigger RNA all the time. And we also used P<sub>C&#8544;</sub> as a constitutive promoter to transcribe switch RNA. If our Toehold switch could work normally, GFP could be translated and we could see green bacteria in our culture medium.
  
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Plasmid profile
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https://2020.igem.org/wiki/images/e/ea/T--NAU-CHINA--composite-parts4.1.png
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 09:01, 11 October 2020


PliaG-B0034-lacI-Pgrac-B0034-CⅠ-PCⅠ-GFP-B0015

We used constitutive promoter PliaG to transcribe trigger RNA all the time. And we also used PCⅠ as a constitutive promoter to transcribe switch RNA. If our Toehold switch could work normally, GFP could be translated and we could see green bacteria in our culture medium.

Plasmid profile T--NAU-CHINA--composite-parts4.1.png Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1294
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2155