Difference between revisions of "Part:BBa K3408006"
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Used Pnar(BBa_K3408000), RBS(BBa_B0034), GFP(BBa_E0040) and terminator(BBa_B0015) to express GFP under the control of P<sub>nar</sub>. When our Bacillus subtilis was in an anerobic environment, Pnar was activated by FNR so that GFP could be expressed. But when our <i>Bacillus subtilis</i> was in an aerobic environment, P<sub>nar</sub> could be suppressed and we couldn't see GFP anymore. | Used Pnar(BBa_K3408000), RBS(BBa_B0034), GFP(BBa_E0040) and terminator(BBa_B0015) to express GFP under the control of P<sub>nar</sub>. When our Bacillus subtilis was in an anerobic environment, Pnar was activated by FNR so that GFP could be expressed. But when our <i>Bacillus subtilis</i> was in an aerobic environment, P<sub>nar</sub> could be suppressed and we couldn't see GFP anymore. | ||
| − | Plasmid | + | Plasmid profile |
https://2020.igem.org/wiki/images/1/18/T--NAU-CHINA--composite-parts1.1.png | https://2020.igem.org/wiki/images/1/18/T--NAU-CHINA--composite-parts1.1.png | ||
Revision as of 08:54, 11 October 2020
Used Pnar(BBa_K3408000), RBS(BBa_B0034), GFP(BBa_E0040) and terminator(BBa_B0015) to express GFP under the control of Pnar. When our Bacillus subtilis was in an anerobic environment, Pnar was activated by FNR so that GFP could be expressed. But when our Bacillus subtilis was in an aerobic environment, Pnar could be suppressed and we couldn't see GFP anymore.
Plasmid profile
