Difference between revisions of "Part:BBa K3394004"

 
Line 7: Line 7:
 
Our cellulolytic complex includes cellulase endo5a linked to CBD (cellulose binding domain) through a GS-linker and a  6-histidine tag (His-tag) at the N-terminus.
 
Our cellulolytic complex includes cellulase endo5a linked to CBD (cellulose binding domain) through a GS-linker and a  6-histidine tag (His-tag) at the N-terminus.
  
−
This complex was designed to be experssed on E.coli's surface through AIDA-1 auto-display system, since cellulose cannot cross the membrane.
+
 
  
  

Revision as of 08:35, 11 October 2020


Cellulolytic complex [1]: His-Endo5a-GS-CBD

Since cellulose cannot cross the membrane, we engineered E. coli to express cellulolytic complex on its surface through AIDA-1 auto-display system.

Our cellulolytic complex includes cellulase endo5a linked to CBD (cellulose binding domain) through a GS-linker and a 6-histidine tag (His-tag) at the N-terminus.



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 988
    Illegal EcoRI site found at 1314
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 988
    Illegal EcoRI site found at 1314
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 988
    Illegal EcoRI site found at 1314
    Illegal BamHI site found at 28
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 988
    Illegal EcoRI site found at 1314
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 988
    Illegal EcoRI site found at 1314
    Illegal AgeI site found at 1289
  • 1000
    COMPATIBLE WITH RFC[1000]