Difference between revisions of "Part:BBa K3380102"

 
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<partinfo>BBa_K3380102 short</partinfo>
 
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F30 downstream RNA aptamer Scaffold is an RNA scaffold that increases the stability and fluorescence of the RNA aptamer once bound to a fluorogen. The F30 scaffold is an engineered version of the naturally occurring phi-29 viral RNA three-way junction motif, which is not cleaved by cellular nucleases.  
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The F30 scaffold is a short RNA sequence that is transcribed into a tRNA scaffold, it can help improve the fluorescence of RNA aptamers such as iSpinach [https://parts.igem.org/Part:BBa_K3380150 BBa_K3380150], DIR2s-Apt [https://parts.igem.org/Part:BBa_K3380151 BBa_K3380151], Malachite Green [https://parts.igem.org/wiki/index.php/Part:BBa_K494000 BBa_K494000] and Broccoli [https://parts.igem.org/wiki/index.php/Part:BBa_K3380153 BBa_K3380153]
  
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===Usage and Biology===
 
===Usage and Biology===
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RNA aptamers can be expressed in cells to influence and image cellular processes. Aptamer folding is maintained by inserting the aptamers into highly structured tRNA scaffolds. The F30 tRNA scaffold, besides facilitating the fluorescent RNA aptamer folding, helps protect it from being degraded by the RNAses.
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Should be used with the upstream F30 scaffold (BBa_K3380101), therefore both scaffolds flanking the RNA aptamer.
  
 
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Latest revision as of 09:06, 10 October 2020


F30 Downstream RNA aptamer scaffold

The F30 scaffold is a short RNA sequence that is transcribed into a tRNA scaffold, it can help improve the fluorescence of RNA aptamers such as iSpinach BBa_K3380150, DIR2s-Apt BBa_K3380151, Malachite Green BBa_K494000 and Broccoli BBa_K3380153

Usage and Biology

RNA aptamers can be expressed in cells to influence and image cellular processes. Aptamer folding is maintained by inserting the aptamers into highly structured tRNA scaffolds. The F30 tRNA scaffold, besides facilitating the fluorescent RNA aptamer folding, helps protect it from being degraded by the RNAses.

Should be used with the upstream F30 scaffold (BBa_K3380101), therefore both scaffolds flanking the RNA aptamer.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]