Difference between revisions of "Part:BBa K3380101"

Revision as of 08:24, 10 October 2020

F30 Upstream RNA aptamer scaffold Short RNA sequence that is transcribed into a tRNA scaffold, it can help improve the fluorescence of RNA aptamers such as iSpinach BBa_K3380150, DIR2s-Apt BBa_K3380151, Malachite Green BBa_K494000 and Broccoli https://parts.igem.org/wiki/index.php/Part:BBa_K3380153 BBa_K3380153]

RNA aptamers can be expressed in cells to influence and image cellular processes. Aptamer folding is maintained by inserting the aptamers into highly structured tRNA scaffolds. The F30 tRNA scaffold, besides facilitating the fluorescent RNA aptamer folding, helps protect it from being degraded by the RNAses.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K3380101 short</partinfo>
-Short RNA sequence that is transcribed into a tRNA scaffold, it can help improve the fluorescence of RNA aptamers such as iSpinach [https://parts.igem.org/Part:BBa_K3380150 BBa_K3380150],
+Short RNA sequence that is transcribed into a tRNA scaffold, it can help improve the fluorescence of RNA aptamers such as iSpinach [https://parts.igem.org/Part:BBa_K3380150 BBa_K3380150], DIR2s-Apt [https://parts.igem.org/Part:BBa_K3380151 BBa_K3380151], Malachite Green [https://parts.igem.org/wiki/index.php/Part:BBa_K494000 BBa_K494000] and Broccoli https://parts.igem.org/wiki/index.php/Part:BBa_K3380153 BBa_K3380153]
 RNA aptamers can be expressed in cells to influence and image cellular processes. Aptamer folding is maintained by inserting the aptamers into highly structured tRNA scaffolds. The F30 tRNA scaffold, besides facilitating the fluorescent RNA aptamer folding, helps protect it from being degraded by the RNAses.