Difference between revisions of "Part:BBa K3504009"

Revision as of 17:42, 9 October 2020

Alphavirus replicon NSPs- Equine Encephalitis virus

Part Description

A composite of parts (BBa_K3504000,BBa_K3504001,BBa_K3504002,BBa_K3504003) and CMV promoter which form ,as a unit, the main constituent of alphavirus replicon which as a whole can give the circuit self-replicating ability

Usage

Characterization

Improvements

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 2760
Illegal SpeI site found at 2708
Illegal PstI site found at 5089
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 2760
Illegal NheI site found at 5960
Illegal NheI site found at 7290
Illegal NheI site found at 8107
Illegal SpeI site found at 2708
Illegal PstI site found at 5089
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 2760
Illegal BglII site found at 2882
Illegal BamHI site found at 2744
Illegal BamHI site found at 3107
Illegal XhoI site found at 6124
Illegal XhoI site found at 6185
Illegal XhoI site found at 6226
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 2760
Illegal SpeI site found at 2708
Illegal PstI site found at 5089
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 2760
Illegal SpeI site found at 2708
Illegal PstI site found at 5089
Illegal NgoMIV site found at 3549
Illegal AgeI site found at 5665
Illegal AgeI site found at 6034
Illegal AgeI site found at 6361
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1635
Illegal BsaI site found at 6223
Illegal BsaI site found at 6241
Illegal BsaI.rc site found at 3012
Illegal BsaI.rc site found at 3659
Illegal BsaI.rc site found at 5750

@@ Line 5: / Line 5: @@
 ==Part Description==
 A composite of parts (BBa_K3504000,BBa_K3504001,BBa_K3504002,BBa_K3504003) and CMV promoter which form ,as a unit, the main constituent of alphavirus replicon which as a whole can give the circuit self-replicating ability
 ==Usage==
 ==Characterization==