Difference between revisions of "Part:BBa K3380153"
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Broccoli is a fluorescent RNA aptamer comprising a short ribonucleotide sequence that exhibits fluorescence upon binding to a specific fluorophore. | Broccoli is a fluorescent RNA aptamer comprising a short ribonucleotide sequence that exhibits fluorescence upon binding to a specific fluorophore. | ||
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+ | ===Characterization=== | ||
+ | The Broccoli secondary structure folding prediction as simulated by [http://www.nupack.org// NUPACK] is shown below. | ||
+ | |||
+ | [[File:Part BBa K3380153.png|400px|Broccoli folding]] | ||
===Usage and Biology=== | ===Usage and Biology=== |
Revision as of 16:26, 8 October 2020
Broccoli fluorescent RNA aptamer
Broccoli is a fluorescent RNA aptamer comprising a short ribonucleotide sequence that exhibits fluorescence upon binding to a specific fluorophore.
Characterization
The Broccoli secondary structure folding prediction as simulated by [http://www.nupack.org// NUPACK] is shown below.
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]