Difference between revisions of "Part:BBa K3408008"

Revision as of 14:26, 8 October 2020

P_nar-B0034-CⅠ-B0015-P_CⅠ-B0034-GFP-B0015

We designed this composite part by combining promoter P_nar, trigger RNA, P_CⅠ, switch RNA and mazF. So we could utilize it to verify the feasibility of our device, which comprises of Toehold switch and suicide protein—mazF. Former composite part BBa_K3408006 has demonstrated the feasibility of promoter P_nar, so when our engineered bacteria Bacillus subtilis is in anaerobic environment, promoter P_nar is activated by global regulator—FNR. Then downstream trigger RNA is expressed. As switch RNA is constantly expressed under the control of promoter P_C1, the trigger RNA can successfully trigger the Toehold switch, thus leading to the expression of mazF. So Bacillus subtilis can commit suicide. While in aerobic environment, promoter P_nar ceases to work, Toehold switch cannot be triggered. As a result, mazF can not express. This device proves that our suicide module and Toehold switch can successfully combine together.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1709

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 <partinfo>BBa_K3408008 short</partinfo>
-We employed Promoter P<sub>nar</sub>, C&#8544; protein, toehold switch comprising of trigger RNA, and Switch RNA to precisely regulate the expression of suicide protein mazF. As a result, when the engineered bacteria reaches the outside aerobic environment, C&#8544; protein ceased to be expressed. Thus Switch RNA is expressed, along with former accumulated Trigger RNA, Toehold switch is triggered. Then our suicide protein mazF is expressed, thus leading to the suicide of our engineered bacteria.
+We designed this composite part by combining promoter P<sub>nar</sub>, trigger RNA, P<sub>CⅠ</sub>, switch RNA and mazF. So we could utilize it to verify the feasibility of our device, which comprises of Toehold switch and suicide protein—mazF. Former composite part BBa_K3408006 has demonstrated the feasibility of promoter P<sub>nar</sub>, so when our engineered bacteria <i>Bacillus subtilis</i> is in anaerobic environment, promoter P<sub>nar</sub> is activated by global regulator—FNR. Then downstream trigger RNA is expressed. As switch RNA is constantly expressed under the control of promoter P<sub>C1</sub>, the trigger RNA can successfully trigger the Toehold switch, thus leading to the expression of mazF. So <i>Bacillus subtilis</i> can commit suicide. While in aerobic environment, promoter P<sub>nar</sub> ceases to work, Toehold switch cannot be triggered. As a result, mazF can not express. This device proves that our suicide module and Toehold switch can successfully combine together.
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