Difference between revisions of "Part:BBa K3408007"
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<partinfo>BBa_K3408007 short</partinfo> | <partinfo>BBa_K3408007 short</partinfo> | ||
− | Used | + | Used P<sub>nar</sub>(BBa_K3408000), RBS(BBa_B0034), optimized CⅠ(BBa_K3408004), terminator(BBa_B0015), P<sub>CⅠ</sub>(BBa_R0051), RBS(BBa_B0034) and GFP(BBa_E0040) to express GFP when PCⅠ was activated. When our <i>Bacillus subtilis</i> was in an aerobic environment, P<sub>nar</sub> was suppressed without FNR and no CⅠ protein could be expressed so that P<sub>CⅠ</sub> could be activated and we could see GFP expressed. But when our <i>Bacillus subtilis</i> was in an anerobic environment, CⅠ protein could be expressed to suppress P<sub>CⅠ</sub> and we couldn't see GFP anymore. |
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Revision as of 12:04, 8 October 2020
Pnar-B0034-phy(ycD)-B0015
Used Pnar(BBa_K3408000), RBS(BBa_B0034), optimized CⅠ(BBa_K3408004), terminator(BBa_B0015), PCⅠ(BBa_R0051), RBS(BBa_B0034) and GFP(BBa_E0040) to express GFP when PCⅠ was activated. When our Bacillus subtilis was in an aerobic environment, Pnar was suppressed without FNR and no CⅠ protein could be expressed so that PCⅠ could be activated and we could see GFP expressed. But when our Bacillus subtilis was in an anerobic environment, CⅠ protein could be expressed to suppress PCⅠ and we couldn't see GFP anymore.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 454
- 1000COMPATIBLE WITH RFC[1000]