Difference between revisions of "Part:BBa K3349007:Design"

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===Design Notes===
 
===Design Notes===
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The part was codon optimized for use in <i>E. coli</i> . The his tag was placed on the C-terminus based on previous pectin lyase studies [1]
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The part was codon optimized for use in <i>E. coli</i>. The his tag was placed on the C-terminus based on previous pectin lyase studies [4].
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===Source===
 
===Source===
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===References===
 
===References===
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1. Mei, Y., Chen, Y., Zhai, R., and Liu, Y. (2013) Cloning, purificationa nd biochemical properties of a thermostable pectinase from <i>Bacillus haldurans</i> M29.
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1. Keggi, C and Doran-Peterson, J. (2020) The homogalacturonan deconstruction system of <i>Paenibacillus amylolyticus</i> 27C64 requires no extracellular Pectin Methylesterase and has significant industrial potential. Applied and environmental microbiology. 86, e02275-19.
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2. Roy, A., Kucukural, A., and Zhang, Y. (2010) I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738.
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3. Waterhouse, A., Bertoni, M., Bienert, S., Studer, G., Tauriello, G., Gumienny, R., Heer, F.T., de Beer, T.A.P., Rempfer, C., Bordoli, L., Lepore, R. and Schwede, T.(2018) SWISS-MODEL: homology modelling of protein structures and complexes. Nucleic Acids Res. 46, W296-W303.
 +
 
 +
4. Mei, Y., Chen, Y., Zhai, R., and Liu, Y. (2013) Cloning, purification and biochemical properties of a thermostable pectinase from <i> Bacillus haldurans</i> M29. Journal of Molecular Catalysis B: Enzymatic. 94, 77-81

Latest revision as of 15:18, 7 October 2020


Pectin Lyase PelB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 333
    Illegal BamHI site found at 1218
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part was codon optimized for use in E. coli. The his tag was placed on the C-terminus based on previous pectin lyase studies [4].

Source

The protein sequence was taken from the Uniprot server. The sequence ID from NCBI is WP_123064018.1.

References

1. Keggi, C and Doran-Peterson, J. (2020) The homogalacturonan deconstruction system of Paenibacillus amylolyticus 27C64 requires no extracellular Pectin Methylesterase and has significant industrial potential. Applied and environmental microbiology. 86, e02275-19.

2. Roy, A., Kucukural, A., and Zhang, Y. (2010) I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738.

3. Waterhouse, A., Bertoni, M., Bienert, S., Studer, G., Tauriello, G., Gumienny, R., Heer, F.T., de Beer, T.A.P., Rempfer, C., Bordoli, L., Lepore, R. and Schwede, T.(2018) SWISS-MODEL: homology modelling of protein structures and complexes. Nucleic Acids Res. 46, W296-W303.

4. Mei, Y., Chen, Y., Zhai, R., and Liu, Y. (2013) Cloning, purification and biochemical properties of a thermostable pectinase from Bacillus haldurans M29. Journal of Molecular Catalysis B: Enzymatic. 94, 77-81