Difference between revisions of "Part:BBa K3349004:Design"

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===Design Notes===
 
===Design Notes===
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The part was codon optimized for use in E. coli . The his tag was placed on the C-terminus of the GFP reporter.  
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The part was codon optimized for use in <i>E. coli</i>. The his tag was placed on the C-terminus of the GFP reporter.
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===Source===
 
===Source===
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===References===
 
===References===
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1. Keggi, C and Doran-Peterson, J. (2020) The homogalacturonan deconstruction system of Paenibacillus amylolyticus 27C64 requires no extracellular Pectin Methylesterase and has significant industrial potential. Applied and environmental microbiology. 86, e02275-19.
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1. Keggi, C and Doran-Peterson, J. (2020) The homogalacturonan deconstruction system of <i>Paenibacillus amylolyticus</i> 27C64 requires no extracellular Pectin Methylesterase and has significant industrial potential. Applied and environmental microbiology. 86, e02275-19.
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2.Roy, A., Kucukural, A., and Zhang, Y. (2010) I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738.
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Latest revision as of 15:11, 7 October 2020


Pectin Lyase PelB-GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 261
    Illegal BamHI site found at 1146
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part was codon optimized for use in E. coli. The his tag was placed on the C-terminus of the GFP reporter.

Source

The pectase sequence was taken from the Uniprot server. The sequence ID from NCBI is WP_123064018.1. The GFP sequence was taken from uniprot as well (P42212)

References

1. Keggi, C and Doran-Peterson, J. (2020) The homogalacturonan deconstruction system of Paenibacillus amylolyticus 27C64 requires no extracellular Pectin Methylesterase and has significant industrial potential. Applied and environmental microbiology. 86, e02275-19.