Revision as of 14:29, 7 October 2020

phyA1-406_N_part

Toulouse_INSA-UPS 2020contributed to the characterisation of this part by adding a new documentation learned form literature on how to use Fhy1 and PhyA as a transcriptoinal optogenetic effectors.
(--antonmykhailiuk 15:26, 07 October 2020 (UTC+2))

• PhyA is an interaction partner of Fhy1 (parts BBa_I757002, I757004)
• Purpose: design of a light switchable interaction
• this part codes for Amino acids 1-406, encompassing the potential Fhy1-interaction domain
• N-terminus of this protein should not be fused for fusions to avoid disturbance of folding and potential Fhy1 interaction ("N-part")
• SwissProt: P14712
• NgoMIV / AgeI protein fusion part
• iGEM Team Freiburg 2007
• synthetic DNA by GeneArt optimized for E.coli
• Part in pGA4 vector (AmpR, ColEI ori)
  

Contribution from other teams

Toulouse_INSA-UPS 2020's contribution

Characterisation

The majority of phytochromes (PhyA to PhyE) in Arabidopsis thaliana are subject to conformational changes induced by light. On the contrary, only PhyA and PhyB were found to have the interacting proteins upon the light illumination.

References

[1]Ni, M., Tepperman, J. M., & Quail, P. H. (1999). Binding of phytochrome B to its nuclear signalling partner PIF3 is reversibly induced by light. Nature, 400(6746), 781–784. https://doi.org/10.1038/23500 [2]Quail, P., Boylan, M., Parks, B., Short, T., Xu, Y., & Wagner, D. (1995). Phytochromes: photosensory perception and signal transduction. Science, 268(5211), 675–680. https://doi.org/10.1126/science.7732376 [3]Kim, J. (2003). Functional Characterization of Phytochrome Interacting Factor 3 in Phytochrome-Mediated Light Signal Transduction. THE PLANT CELL ONLINE, 15(10), 2399–2407. https://doi.org/10.1105/tpc.014498

Sequence and Features