Difference between revisions of "Part:BBa I757007"
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| − | [https://2020.igem.org/Team:Toulouse_INSA-UPS] contributed to the characterisation of this part by adding a new documentation learned form literature on how to use Fhy1 and PhyA as a transcriptoinal optogenetic effectors. <br> | + | [https://2020.igem.org/Team:Toulouse_INSA-UPS Toulouse_INSA-UPS 2020]contributed to the characterisation of this part by adding a new documentation learned form literature on how to use Fhy1 and PhyA as a transcriptoinal optogenetic effectors. <br> |
| − | (<small>--[[User:antonmykhailiuk|antonmykhailiuk]] 15:26, 07 October 2020 (UTC+2)</small>) | + | (<small>--[[User:antonmykhailiuk|antonmykhailiuk]] 15:26, 07 October 2020 (UTC+2)</small>)<br> |
* PhyA is an interaction partner of Fhy1 (parts BBa_I757002, I757004) | * PhyA is an interaction partner of Fhy1 (parts BBa_I757002, I757004) | ||
| Line 14: | Line 14: | ||
* iGEM Team Freiburg 2007 | * iGEM Team Freiburg 2007 | ||
* synthetic DNA by GeneArt optimized for E.coli | * synthetic DNA by GeneArt optimized for E.coli | ||
| − | * Part in pGA4 vector (AmpR, ColEI ori) | + | * Part in pGA4 vector (AmpR, ColEI ori)<br> |
| − | < | + | <h1>Contribution from other teams</h1> |
| − | ===Characterisation=== | + | |
| + | ==Toulouse_INSA-UPS 2020's contribution== | ||
| + | ===''Characterisation''=== | ||
| + | <p style="text-indent: 40px"> | ||
Revision as of 14:00, 7 October 2020
phyA1-406_N_part
Toulouse_INSA-UPS 2020contributed to the characterisation of this part by adding a new documentation learned form literature on how to use Fhy1 and PhyA as a transcriptoinal optogenetic effectors.
(--antonmykhailiuk 15:26, 07 October 2020 (UTC+2))
- PhyA is an interaction partner of Fhy1 (parts BBa_I757002, I757004)
- Purpose: design of a light switchable interaction
- this part codes for Amino acids 1-406, encompassing the potential Fhy1-interaction domain
- N-terminus of this protein should not be fused for fusions to avoid disturbance of folding and potential Fhy1 interaction ("N-part")
- SwissProt: P14712
- NgoMIV / AgeI protein fusion part
- iGEM Team Freiburg 2007
- synthetic DNA by GeneArt optimized for E.coli
- Part in pGA4 vector (AmpR, ColEI ori)
Contribution from other teams
Toulouse_INSA-UPS 2020's contribution
Characterisation
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1219
- 1000COMPATIBLE WITH RFC[1000]